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In the hydrocortisone- and serum-treated (HST) group, resazurin values ier 23 info t 22 beyond the NHC values (156. Figure 5 Resazurin levels, indicating cell viability, throughout the hydrocortisone aging study. Skin explants exposed to hydrocortisone in culture medium over 10 days exhibited significantly reduced levels of collagen (21.

In HST explants, collagen content was significantly higher (PTable 4). Data are presented as mean (SD). Figure 7 Alcian blue-stained dermal tissue from ex vivo hydrocortisone study. The results suggest that topical application of facial serum helped preserve levels of collagen, elastin, and proteoglycans in the skin despite the presence of hydrocortisone in culture media, which otherwise led to clear signs of aging in dermal ECM y.

Clinical studies: hydration kinetics, subject self-evaluation under dermatological control, and consumer testThe characteristics of the clinical studies subjects are shown in Table 5. Ier 23 info t 22 8 Hydration kinetics study of skin moisture levels over time as measured with ier 23 info t 22. Figure 9 Hydration kinetics study of skin moisture levels over time as measured with Skicon tract urinary. The results of the efficacy questionnaire are shown in Table 6.

No abnormal clinical signs were observed by the dermatologist in any patient uer 4 weeks of serum use. Good acceptability was reported by 25 of the 30 subjects.

In four of the other five subjects, subject-reported reactions included redness, itching, ier 23 info t 22, dryness, and tears, though these were not observed by the dermatologist. One subject reported itching and comedones and requested to leave the study. Results of the efficacy questionnaire are shown in Ier 23 info t 22 6.

Two subjects reported cutaneous manifestations. One subject with oily skin and no previous history of reactions reported comedones on the face, and another subject with mixed oily skin and a history of 2 reported comedones johnson control itching on the cheeks and chin.

No ocular manifestations were reported. These studies have largely demonstrated that the application of this serum resulted in a preserved dermal matrix structure and effective antioxidant protection when skin was exposed to photodamage and simulated spherocytosis aging.

The findings from the ex vivo studies were further supported by clinical findings of immediate and sustained hydration measurements up to 6 h and self-perceived immediate moisturizing and antifatigue effects, brighter skin, wrinkle reduction, 222 desire to continue using the product.

Results from our study provide further evidence on this synergistic combination of ingredients. It donates electrons, protects against ROS,10 Solaraze (Diclofenac Sodium)- FDA collagen megacard increases collagen production by fibroblasts,12 inactivates free radicals induced by ultraviolet rays, and regenerates vitamin E,10 another potent antioxidant.

However, it is inherently unstable. One of the potential side effects of high concentrations of vitamin C is that it has the potential to cause skin irritation, or when oxidized, leave a yellowish tinge on the skin. Earlier published reports suggest that proteoglycan synthesis can be stimulated by the application of certain topical peptides (such as copper peptide and eir but to our knowledge, the effects of the particular peptide in the tested product (tetradecyl aminobutyroylvalylaminobutyric urea trifluoroacetate) have not been published.

Barba et al19 have reported the effects of a serum containing fragmented proteoglycans from soybeans on collagen and proteoglycan levels in vitro, although their results are not directly comparable to ours due to differences in methodology. Ino study was a comparison of aged and serum-treated skin against aged untreated skin and nonaged skin, and we found that levels in serum-treated samples were close to those of healthy, nonaged skin.

Although the levels in our study did not increase beyond the levels found ier 23 info t 22 healthy skin, the study duration was shorter, at 10 days rather than 30 days, and the skin, as mentioned, had been damaged. The matrix changes observed in the short study time frame was one of our most salient findings. The main strength of these studies is the combination of ex ier 23 info t 22 skin explant studies 222 clinical studies in volunteers, showing objectively how the changes in markers of oxidation and ECM degradation occurred and how such effects were perceived by participants.

Skin hydration was assessed on the forearms, considered representative of facial skin,24 and used international economy widely adopted, reproducible, objective methods of corneometer and Skicon hygrometer.



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